EVALUATION OF HSP 70/90 LEVELS IN PREGNANT WOMEN WITH MILD PREECLAMPSIA

Abstract

Preeclampsia is still one of the leading causes of maternal and perinatal mortality and morbidity worldwide. ). Overexpression of HSPs is induced by many types of cell stress, such as oxidative stress, inflammation, and toxic compounds. HSP 70 and HSP 90 have important roles in the regulation of the cell’s life process, cell functions, and continuousness of immunity. In this study, we aimed to investigate HSP 70/90 expressions in mild preeclampsia groups. 30 pregnancy were included in the study, 15 were control and 15 were mild preeclampsia at Gaziantep University Şahinbey Research and Application Hospital Department of Obstetrics and Gynecology. As the control group, umbilical tissues taken from 15 women who did not have any metabolic disease were used. Mild preeclampsia (MP) was considered if systolic blood pressure was greater than 140 mmHg or diastolic blood pressure greater than 90 mmHg and proteinuria more than 4g in a 24-hour urine sample and their umbilical tissues were collected to examine them in experimental stages. The umbilical cords of pregnant women were taken during delivery for immünohistochemia and western blot. HSP 70/90 antibodies were examined using western blot. Also, HSP 70/90 expression was evaluated by immunohistochemistry method. Western blot and immunohistochemical methods were carried out in experimental stages. The western blot and immunohistochemia analyses showed that the expressions of the proteins HSP 70/90 were significantly higher in the umbilical cords of women with mild preeclampsia than in the umbilical cords of control women. The relationship between HSP 70/90 and PE is stil controversial. These data suggest that upregulated HSP 70/90 levels were probably associated with increased apoptosis in the umbilical tissues of PE patients and may contribute to the pathophysiology of PE.